Research on Conservation of Dendrobium Candidum Capsule in Vitro
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Graphical Abstract
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Abstract
Using the hybrid capsule of Dendrobium candidumas the material, the germination and perimeter of its seeds were studied in different preservation conditions in order to find out about the suitable conditions for vitro conservation. The result showed that the optimal conservation condition is when it is kept for 30 d, 60 d and 150 d under the temperature of 4℃ in refrigerator. The seeds is found to have an average germination rate of 81.77% when it is planted in the substrate of 1/2MS + NAA0.2 mg/L+ potato juice 150 g/L. After 10 of germination, the perimeter is larger, compared with the effect by other treating methods. It proves that such a method can be used as a method for short-term preservation of Dendrobium Candidum Capsule in Vitro after harvest.
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