Abstract: To enhance the students capacity in independent study,independent experiments were designed. Discussions were principally based on the independent experiment practice in microbiology during 2011～2012, in which the sophomores of three different majors in the College of Life Science; and details for the selection of the teaching aim, choice of difficult points and important points, the design strategy and the fulfillment of objectives are all put under examination. To make sure the student can finish their independent experiment and conduct it well; basic skills should be taught and practice be placed first. So it was strongly recommended that the two basic experiments include one comprehensive experiment, which needs to be practiced first under the guidance of the teacher, and one independently designed experiment, which is chosen as the major experiment for both practicing and testing the talent of the students.