Abstract: Termitomyces Heim were cultured in liquid media, at different pH value condition and in different schemes of media, which is designed to find out the best liquid culture conditions. Corn powder, wheat bran, soybean powder, K₂HPO₄, MgSO₄ were selected by the experiments as carbon source, nitrogen source and trace nutrient factor respectively. Experiment was conducted by use of four-factor and four-level orthogonal table. And the dry weight of the mycelia was used as the benchmark biomass. The results showed the best media being: wheat bran14 g/L + corn flour 11 g/L + soybean powder 2 g/L +KH₂PO₄ 1.2 g/L+MgSO₄ 0.9 g/L; besides, the optimum conditions of pH value was 6.0.