Abstract: To investigate the effect of cordyceps militaris extract and amino acids on exercise-induced fatigue and endurance to hypoxia in mice, a model of exercise -induced fatigue in mice was established. After being gavaged with cordyceps militaris extract and amino acids，the time of swim-exhausted exercise，climbing pole, endurance to hypoxia and the change of lactic acid in blood were measured for the mice. Results showed that exercise-exhausted mice, when gavaged with cordyceps militaris extract and amino acids, gave a significantly better performance in respects of the time of swim-exhausted exercise，climbing pole and endurance to hypoxia than when gavaged with saline（P<0.05）. The change of lactic acid in the blood of the mice gavaged with cordyceps militaris extract and amino acids, being the same as that of the mice gavaged with merely cordyceps militaris extract, was significantly lower than that of the mice gavaged with saline or amino acids respectively（P<0.05）. In Conclusion, Cordyceps militaris extract in combination with amino acids can actively decrease exercise-induced fatigue and improve sports physiological function in mice.