Abstract: With eleocharis tuberosa peel as the raw material, the polysaccharide was extracted fromeleocharis tuberosa peel by ultrasonic-assisted pectinase method.The pH, ultrasonic power, ultrasonic treatment temperature, ultrasonic treatment time, enzymatic dosage, liquid-solid ratio, refluxing extraction temperature, and refluxing extraction time, were optimized by single-factor experiments and orthogonal tests.The results showed that the optimal extraction conditions were when the pH value was set at 5, ultrasonic power at 320 W, ultrasonic treatment temperature at 60℃, ultrasonic treatment time for40 min, enzymatic dosage at 0.7%, liquid-solid ratio at 20∶1 mL/g, refluxing extraction temperature at 50℃, and refluxing extraction time for 3 h.Under the optimal conditions, the yield of polysaccharide could reach 24.36%, and with a rather nice reproducibility.The findings provided an experimental method for the further utilization and development of the polysaccharide in eleocharis tuberosa peel.