Abstract: The promotion of 0.07%, 0.10% and 0.13% chitin solution on growth of Dendrobium officinate Kimura et Migo, along with chitin solution on growth of Dendrobium officinate Kimura et Migo in different season were researched in these experiments, in which T4, W3, T2 biennial seedlings were used as test materials. The results showed that the optimistic promotion concentration of the three chitin solution on T4 variety was 0.10%. 0.10% chitin solution has different promotion in different seasons on the growth of Dendrobium officinate Kimura et Migo. The order of growth increment of three indicators in early summer was T₄>W₃>T₂, which indicates the application of chitin in early summer was more effective to promote growth for T₄ than that of other varieties. The order of growth increment of three indicators in high summer was T₂>W₃>T₄, indicating that the application of chitin in high summer was more effective to promote growth for T₂ than that of other varieties. In addition to the T₄ variety, the effect of applying chitin solution in high summer was superior to the early summer. Base on the above experiments the technological data could be referred to as the basis for effective cultivationof Dendrobium officinate Kimura et Migo.