Abstract: The effects of different culture medium, growth hormone proportion, subculture cycle and concentration of active carbon on multiplication of adventitious buds, rooting of Dendrobium flexicaule Z.H.Tsi, S.C.Sun et L.G.Xu were researched in this experiment, in which the tissue culture seedlings were used as material for experiment.The results showed that the multiplication of adventitious buds was relatively satisfactory when the tissue culture seedlings were cultured in medium of B5+0.3 mg/L 6-BA +0.03 mg/L NAA +6 g/L Agar+30 g/L Sugar.In the subculture cycle experiment of 30 d, 50 dand 70 d, subculture cycle of 50 dwas best in favor of multiplication of adventitious buds.When the medium was added with0.30 mg/L NAA and 0.8 g/L activated carbon, it drastically promoted the rooting of tissue culture seedlings in Dendrobium flexicaule Z.H.Tsi, S.C.Sun et L.G.Xu.The above experimental results statistically provided a technical basis for largescale production of Dendrobium flexicaule Z.H.Tsi, S.C.Sun et L.G.Xu.