Abstract: A status quo survey is conducted to find out about the tissue culture of Nyssaceae plants concerning the problems of the choice, treatment and sterilization of exophyte, the types of culture medium and the growth conditioning agent. In the tissue culture of Nyssaceae plants, mature embryo, sprout, section of stem and stem tip are usually used as the exophyte. By means of low temperature, preincubation, running water flush and surface sterilization by spirit and mercury bichloride, successful inducement of adventitious bud and callus can be achieved; in the culture medium of low salt-concentration plus cytokinin and auxin of certain concentration, reproduction of a new plant can be made possible. In addition, existing problems and future developing trend are discussed so as to provide some reference for future research of tissue culture of Nyssaceae plants.