Abstract: The effects of acclimatization training, different medium and detopping treatment on the survival rate and growth of tissue culture seedlings of Bletilla striata( Thunb.)Reichb.f.were investigated in the experiment.The results showed that the survival ratio and growth of tissue culture seedlings of Bletilla striata( Thunb.)Reichb.f.were rather satisfactory when they are subjected to the detopping treatment, the acclimatization training of 6 d, using sphagnum and perlite as the organic and non-organic media and the nutrition soil(1/1), which can serve as the technical support for future large-scale cultivation of tissue culture seedlings of Bletilla striata(Thunb.)Reichb.F.